Manic Botanix Connoisseur Blooms Explained
How it Works – Excellence in Hydroponics
The application of plant growth substances to aid setting, fruit size and/or ripening has attracted an immense amount of research with some spectacular successes. Most have originated from empirical testing but a few derive from findings in studies.
These growth substances include safe plant growth stimulants such as Triacontanol (a growth stimulant which is naturally occurring in alfalfa) and Brassinosteroids, a new 6th generation plant growth hormone known as a steroidal plant hormone that naturally occurs in plants.
“Med Safe” Plant Growth Stimulants
The ideal plant growth regulator or stimulant should leave no harmful persistent residue in a finished product or crop and the paradigm compounds are ones that have high specific activity, are target specific, are environmentally biodegradable, and are non harmful to consumers. The most acceptable growth regulators appear to be those compounds that already occur in nature and elicit certain desirable responses. Both Brassinosteroids and Triacontanol fall within this group.
What’s in Manix Connoisseur Blooms?
Triacontanol (TRIA) has been realized as a potent plant growth promoting substance for a number of agricultural and horticultural crops.1
Triacontanol can be applied to the plant during any stage of growth, from seed or cutting to harvest day. Triacontanol is non-toxic to plants, animals, and humans at all levels within reason and is safe to use on consumable crops. Triacontanol can be co-applied with Auxins, Gibberellins, Cytokinins, and Brassinosteroids.
1-Triacontanol is a fatty alcohol of the general formula C30H62O, also known as melissyl alcohol or myricyl alcohol. It is found in plant cuticle waxes and in beeswax. Triacontanol is a plant growth regulator in the subclass of “growth stimulant” shown to increase yields in many plants – most notably C3 plants. 2
C3 plants include wheat, rice, daisies, petunias, roses, fruit trees, conifers and cannabis. ALL C3 plants can benefit from TRIA applications, regardless of growing style or environmental conditions, although different types of C3 plants will have different optimum dosage rates of TRIA. Many investigators have shown that TRIA affects several basic metabolic processes including photosynthesis, nutrient uptake, and enzyme activity. 3
Triacontanol has shown the ability to (somewhat) alleviate negative effects of stress induced by salinity toxicity, cold temperatures, and CO2 and light deprivation.
Applications of Triacontanol have been shown to increase both water and nutrient uptake, CO2 fixation, endogenous levels of Adenosine triphosphate (essential units of energy for all life), Rubisco Activase (often the limiting factor in C3 photosynthesis), chlorophyll a & b content and increased essential oil content of plants (not relative to trichome density).
Triacontanol applied to tomato plants as a foliar spray caused a significant increase in total yield and yield per plant. When triacontantol was added to the growth medium, only a temporary increase in yield and number of fruits was observed.
TRIA applied as a foliar spray to tomato plants increased the total yield by 12% and the number of fruits from all plants by 25% as compared to the control group. However, TRIA added to the growth medium increased total yield by only 6% and the number of fruits by 3%. 4
In research conducted by D. Skogen, et al (1981) two cultivars of Chrysanthemum morifolium, ‘Golden Horim’ and ‘Golden Miquel’, were cultivated in nutrient solution containing the growth regulator triacontanol. The dry weight of the whole plant and the shoot from both cultivars increased. The number of ‘inflorescences’ (a group of flowers growing from a common stem, often in a characteristic arrangement. Also called flower cluster) per plant and the number of flowers per inflorescence also increased in response to triacontanol treatment, which in turn enhanced the quality of flowers. The number of flowers of superior quality was more than doubled.5
While research conducted by N. K. Srivastava et al (1989) on Opium Poppies treated with Triacontanol via foliar application demonstrated:
“Plant height, capsule number and weight, morphine content, CO2 exchange rate, total chlorophyll and fresh and dry weight of the shoot were significantly maximum at 0 .01 mg/1 Tria. At the highest concentration (4mg/1) total chlorophyll, CO2 exchange rate and plant height were significantly inhibited. Thebaine (a crystalline, poisonous, and anodyne alkaloid from opium) and codeine contents remained unaffected at all the concentrations. The concentration of Fe, Mn, Cu in shoots were maximum at .01 and Zn at 0 .1 mg/l Tria. Increase in shoot weight, leaf area ratio and chlorophyll content were significantly correlated with morphine content…
The present investigation reveals that Tria at concentrations upto 0 .1 mg/1 significantly enhances various processes related to production physiology
in opium poppy . The primary processes in turn contribute significantly in
increasing overall yield of straw, capsule and morphine content .” 6
In trials conducted on essential oil bearing plants (mint) by M. Naeem et al (2011) findings showed:
“Out of a large number of essential oil bearing plants, mint (Mentha arvensis L.) constitutes the most important source of therapeutic agents used in the alternative systems of medicine. The mint plant has marvelous medicinal properties. In view of enhancing growth, yield and quality of this medicinally important plant, a pot experiment was conducted according to simple randomized block design. The experiment was aimed at studying the effect of four concentrations of TRIA (10-0, 10-7, 10-6 and 10-5 M) on the performance of mint with regard to growth and other physiological attributes, crop yield and quality attributes and the yield and contents of active constituents of the plant. The growth and other physiological parameters as well as yield and quality attributes were studied at 100 and 120 DAP. The foliar application of TRIA at 10-6 M concentration significantly enhanced most of the growth and other physiological attributes, crop herbage yield and the yield and content of active constituents (menthol, L-methone, isomenthone and menthyl acetate) of mint at both the stages. However, the next higher concentration of TRIA (10-5 M) exhibited slightly negative effect and did not further increase the values of the attributes studied, but it proved significantly better than the control. Application of TRIA significantly enhanced the yield and content of all the active constituents… “ 7
The only known negative side effect from TRIA other than over application is that it can suppress certain defence mechanisms that help ward off insect infestation. TRIA suppresses the production of certain proteinase inhibitors that are a main defensive mechanism against insect infestation. More often than not this effect is not noticeable, but we do not suggest applying TRIA to any plants that are having issues with an insect infestation.
Further to this, several factors can reduce the effectiveness of TRIA as a growth stimulator. Inhibitory compounds, which have been reviewed in detail, include long chain alcohols, morpholine (commonly found in distilled water from steam condensates), and phthalate esters, particularly from polyvinyl chloride tubing.8
When applying Triacontanol to the plant, it’s best to apply it to the foliage (as is shown in numerous studies). Foliar applications of TRIA consistently have better improvements in growth and allow less use than applications of TRIA to the growth media. For instance, in research conducted by A. B. Eriksen et al (1982) Triacontanol applied to tomato plants as a foliar spray caused a significant increase in total yield and yield per plant. When triacontantol was added to the growth medium, only a temporary increase in yield and number of fruits was observed.
The best time to apply any products to the foliage of your plant is the beginning of the night cycle for your plants to allow minimum evaporation of your foliar spray. If growing indoors be sure to turn all fans off for a minimum of 6 hours, preferably until all leaves are dry. If growing outdoors try to apply spray on a night where there will be little wind. The higher the humidity the longer the spray will stay on the leaves and the better the penetration through the leaf cuticles will be.
Never foliar feed when lights are on as this can burn the plants.
Brassinosteroids are of universal occurrence in plants. They have been reported to affect plant growth and development through a spectrum of physiological responses.
Large-scale field trials over 6 years have demonstrated significant effect of 24-epibrassinolide, a newly developed and far more stable alternative to brassinolide, on the production of crop and vegetables. More than 10 % increase in the yield of wheat, corn, tobacco, watermelon, and cucumber has been shown. 1
Brassinosteroids (BRs) are naturally occurring plant growth regulators similar to animal steroid hormones that can function as growth regulators. Application of BRs affects a broad spectrum of physiological responses in plants such as cell expansion, Increased C02 assimilation and (hence) increased photosynthesis, vascular differentiation, reproductive development, seed germination, flowering, and fruit set in plants. Apart from growth-promoting effects, BRs are also reported to protect plants against various abiotic (The abiotic factors of the environment include light, temperature, and atmospheric gases) and biotic (e.g. secretions, wastes, and remains) stresses like heat, drought, heavy metals, infections, pesticides, salt, and even viruses. Additionally, some data suggests that exogenous (applied) BRs may promote early ripening (via increases in ethylene levels) in tomato, a climacteric fruit;2 while other data demonstrates increased Brix (sugar) levels as a result of exogenous BRs.3 In research conducted by K. N. Swamy et al, The effects of 24-epibrassinolide on growth, photosynthesis, chlorophyll content, carbohydrate fractions, and essential oil content of rose scented geranium were investigated. Foliar application of 24-epibrassinolide substantially increased growth. Exogenous application of 24-epibrassinolide increased the rate of photosynthesis. Growth promotion was also associated with increased chlorophyll content and resulted in the accumulation of carbohydrates. 24-epibrassinolide increased the total content of essential oils.4
Application of brassinosteroids to cucumbers was demonstrated to increase the metabolism and removal of pesticides, which could be beneficial for reducing the human ingestion of residual pesticides from non-organically grown vegetables.5
Increased Photosynthesis and Calvin Cycle
The effects of 24‐epibrassinolide (EBR) spray application on gas‐exchange, chlorophyll fluorescence characteristics, Rubisco (in simple terms “Rubisco” catalyzes the incorporation of CO2) activity, and carbohydrate metabolism were investigated in cucumber (Cucumis sativus L. cv. Jinchun No. 3) plants grown in a greenhouse. EBR significantly increased the light‐saturated net CO2 assimilation rate (Asat) from 3 h to 7d after spraying. In addition, significant increases in the initial activity of Rubisco and in the sucrose, soluble sugars, and starch contents were observed followed by substantial increases in sucrose phosphate synthase (SPS), sucrose synthase (SS), and acid invertase (AI) activities after EBR treatment. It was concluded that EBR increases the capacity of CO2 assimilation in the Calvin cycle.6
Carbohydrates, Amino Acids, and Sugars
The other key thing Manix Connoisseur Blooms does is increase nutrient availability, boost yields, and sweeten fruit via the use of amino acids, sugars, vitamins and carbohydrates.
Ingredients in our Connoisseur Blooms formulation were carefully selected on the basis that they have low molecular weight and high bioavailabilty. For instance, only simple sugars are able to penetrate the roots Casparian Strip for utilization by the plant. Similarly, carbohydrates that are exogenously applied via nutrients (drench) need to be of a very specific form and even here only a fraction of these carbohydrates can be utilized by the plant. Manix Connoisseur Blooms uses totally organic sugars, flavenoids and carbs – In fact, research shows plants best absorb simple or complex carbohydrates when they are present as dextrose, glucose, maltose and xylose from raw cane extract, malt extract, sulfate free molasses, cranberry extract and other premium ingredients.
Feeding your plants simple sugars, carbohydrates and amino supplements has several advantages. In fact many simple and complex carbohydrates serve as food for the friendly bacteria and fungi in your root zone. Roots constantly exude simple and complex carbohydrates, amino acids, and proteins into the rhizospere and these exudates serve as food and growth promoting compounds for many of the beneficial bacteria and fungi present in your plant/s rhizoshpere.
In turn, these rhizospheric bacteria and fungi form a symbiotic relationship with the plant and in doing so receive food from the plant (exudates such as sugars, amino acids, and complex carbohydrates that the plants release via the roots) and break down these elements in the rhizosphere into ionic forms the plant can absorb as well as excreting protein and secondary signalling molecules of their own that benefit the plant by increasing its rate of growth and development. Nature at its very best where symbiosis aids biomass and yields. Beneficial bacteria and fungi increase root mass, increase nutrient absorption, protect roots, create enzymes (e.g. Trichoderma create cellulose enzymes which help break down dead root matter), and produce hormones that stimulate floral size, and potency. Read more about beneficial microbes in hydroponics here.
About Amino Acids
Amino Acids are fundamental ingredients in the process of protein synthesis. About 20 important amino acids are involved in the process of each function. Studies have shown that amino acids can directly or indirectly influence the physiological activities of the plant. Amino acids can be supplied to plant by incorporating them into the soil or hydroponic substrate. It helps in improving the microflora of the soil or substrate, thereby facilitating the assimilation of nutrients.
Recent research has proven that higher plants can uptake and utilize amino acids via the roots. One study (A. El-Naggar et al, 2009) investigated the uptake of multiple amino acids by plant roots. Under minimum microbial activity conditions, seedlings of wheat (Triticum aestivum L. cv. ‘Baldus’) – a C3 plant – were exposed to a series of different concentrations of seven mixed amino acid solutions. Samples of the depleted solutions were periodically collected over a period of ten hours to measure the concentration of amino acids. For all tested amino acids high degrees of uptake was observed. 1
Additionally, plants synthesize amino acids from the primary elements, the carbon and oxygen obtained from air, hydrogen from water in the soil or nutrients, forming Carbon Hydrate by means of photosynthesis and combining it with the nitrogen, leading to synthesis of amino acids, via metabolic pathways. Only L-Amino acids have metabolic activity in plants. The requirement of amino acids in essential quantities is well known as a means to increase yield and overall quality of crops. The application of amino acids via foliar use (where they are able to be readilly absorbed by the leaves) is based on the requirement of plants in general and at critical stages of growth in particular. Exogenously applied amino acids are shown in numerous studies to benefit yields.
Amino Acids Contained in Manix Connoisseur Blooms:
L – Methioine
Manix Connoisseur Blooms is Not a Nutrient
Manix Connoisseur Blooms is a highly specialized formulation and should be used in conjunction with your standard nutrient regime and a PK booster during flower. For best results, use in conjunction with beneficial bacteria and/or fungi and foliar feed every seven days (along with maintaining Connoisseur Blooms in soltution at 2ml/L).
Manix Botanix Connoisseur Blooms contains:
Hydrolyzed Whey Protein (a rich source of amino acids commonly used by body builders)
Malt Extract Powder
Premium Grade, Sulphate Free, Molasses (Australian sourced and modified in our own laboratory, specifically for use in hydroponics)
Anionic Polyelectrolyte Polymers
Proprietary Dispersion Agents
Proprietary Delivery Agents
1. M. Naeem, M. Masroor A. Khan, Moinuddin, Mohd. Idrees, Tariq Aftab (2011) Triacontanol-mediated regulation of growth and other physiological attributes, active constituents and yield of Mentha arvensis L.
2. B. Eriksen, M. K. Haugstad and S. Nilsen (1982) Yield of tomato and maize in response to foliar and root applications of triacontanol
3. Stanley Ries (1990) Triacontanol and Its Second Messenger9-b-L (+)-Adenosine as Plant Growth Substances
4. A. B. Eriksen, M. K. Haugstad and S. Nilsen (1982) Yield of tomato and maize in response to foliar and root applications of triacontanol
5. D. Skogen, A.B. Eriksen, S. Nilsen (1981) Effect of triacontanol on production and quality of flowers of Chrysanthemum morifolium Ramat
6. N .K. SRIVASTAVA** & SRIKANT SHARMA (1989) Effect of Triacontanol on photosynthesis, alkaloid content and growth in opium poppy (Papaver Somniferum L)
7. Naeem, M.; Khan, M; Moinuddin; Idrees, Mohd; Aftab, Tariq (2011) Triacontanol-mediated regulation of growth and other physiological attributes, active constituents and yield of Mentha arvensis L.
8. Ries SK (1985) Regulation of plant growth with triacontanol.
9. Stanley Ries (1990) Triacontanol and Its Second Messenger 9-b-L (+)-Adenosine as Plant Growth Substances
- Nobuo Ikekawa and , Yu-Ju Zhao2 (1991) Application of 24-Epibrassinolide in Agriculture)
- Gregory M. Symons,, Christopher Davies, Yuri Shavrukov, Ian B. Dry, James B. Reid* and Mark R. Thomas (2006) Grapes on Steroids. Brassinosteroids Are Involved in Grape Berry Ripening.
- K. N. Swamy and S. S. R. Rao (2009) Effect of 24-epibrassinolide on growth, photosynthesis, and essential oil content of Pelargonium graveolens (L.) Herit
- Xiao Jian, Xia; Zhang, Y; Wu, JX; Wang, JT; Zhou, YH; Shi, K; Yu, YL; Yu, JQ (2009). “Brassinosteroids Promote Metabolism of Pesticides in Cucumber”.
- A role for brassinosteroids in the regulation of photosynthesis in Cucumis sativus (2004) Jing Quan Yu, Li Feng Huang, Wen Hai Hu, Yan Hong Zhou, Wei Hua Mao, Su Feng Ye and Salvador Nogués
El-Naggar, A. de Neergaard, A. El-Araby & H. Hgh-Jensen (2009) Simultaneous Uptake of Multiple Amino Acids by Wheat